Fresh RT 5.0.1 Install, 'Can't get LDAP Authentication Working

Just to confirm you do have CN=binduser,CN=Users,DC=amplebay,DC=net” as the bind user in your RTSiteConfig.pm, not, CN=binduser,DC=users,DC=example,DC=net? And the base is set to cn=users,dc=amplebay,dc=net?

I’ve also had issues in the past with being able to construct outgoing network connections from Apache webservers due to default security policies (on CentOS Linux for example). You might want to check that’s OK, especially if SELinux is turned on. Something like setsebool -P httpd_can_network_connect 1 might help.